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Video 2
Combined widefield epifluorescence (pseudocolored red) and TIRFM (pseudocolored green) analysis of GFP-Rab21-transfected MDA-MB-231 American Type Culture Collection breast adenocarcinoma cells. Video is showing bidirectional movement between plasma membrane and cell body. Frames were collected at 0.6-s intervals and are displayed at 30 frames/s. Quantitative plots and still images can be found in Fig. 4 B.