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Figure S3. asl mutant brains contain supernumerary central brain NBs. (A) asl mutant NBs fixed and stained for tubulin and γtub, revealing lack of centrosomes. Arrowheads indicate location of spindle poles. Bar, 10 µm. (B) WT brain. Yellow arrowheads indicate normal NBs. (C) asl mutant brains. Note increase in NB number as a result of occasional symmetric divisions. Yellow arrowheads indicate ectopic paired NBs of similar size, which are never seen in WT. We suspect these originate from symmetric divisions. Bar, 50 µm.