Folding and organization of a contiguous chromosome region according to the gene distribution pattern in primary genomic sequence -- Data Supplement - JCB--Shopland et al. -- The Journal of Cell Biology

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Figure S1. Maps of BAC probes for Mmu14 and Mmu15. (A) BAC clones used to probe the Mmu14 region. Using Ensembl and Celera mouse genome browsers, we expanded an existing tiling path of BACs (Peterson et al., 2002) to span each gene cluster (red) and desert (green) between Tbc1d4 and Spry2. These left a 50-100-kb gap between each segment to allow the clear resolution of each segment in nuclei when probed by FISH (Lawrence et al., 1990). Locations are based on Mouse Genome Build 34 (http://oct2005.archive.ensembl.org/Mus_musculus/index.html). (B) Tiling path of BACs across the gene-rich region of Mmu15 from Myh9 to Tcf20, which was used for FISH probes according to the indicated labeling scheme (red and green boxes indicate different labels on each BAC pool). This region contains an average of 28 genes/Mb and only two gaps between genes, which are >100 kb (140 and 120 kb). All BAC identities were confirmed by interphase FISH mapping in primary MEFs.