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Figure S2. Condensin recruitment and UCC develop in HeLa cells as a delayed cellular response to severe DNA damage. (A) The development of the UCC is closely correlated in time with the recruitment of condensin core proteins to damaged chromatin. Exponentially growing HeLa cells were treated with 200 ng/ml NCS for periods ranging from 4 to 24 h. Red, SMC2 or SMC4; green, DNA (Yo-Pro-1). Bars, 20 µm. (B) UCC and recruitment of condensin subunits to damaged chromatin are dose dependent. HeLa cells were treated with different doses of NCS for 24 h and immunostained with anti-SMC2 or anti-SMC4 antibodies (red). DNA was counterstained with Yo-Pro-1 (green). Bars, 20 µm.