Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage -- Data Supplement - JCB--Blank et al. -- The Journal of Cell Biology

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Figure S3. ATM knockdown does not alter the rate of DSB-induced UCC. (A) HeLa cells stably expressing shRNA against ATM (shATM) or against lacZ (shLacZ) were treated with 200 ng/ml NCS for 24 h, fixed, and immunostained with an anti-SMC2 antibody (red). DNA was counterstained with Yo-Pro-1 (green). Bars, 20 µm. (B) Western blotting analysis demonstrating the degree of ATM knockdown in the cells expressing shRNA against this protein.