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Figure S5. Defective p53-mediated G2/M checkpoint is important for condensin recruitment, UCC, and MCD but is not the sole requirement. (A) Expression levels of p53 and p21waf1 in a panel of human cell lines bearing wild-type p53 or compromised p53. DNA damage was induced with 200 ng/ml NCS. Hsc70 was used as a loading control. (B) Condensin recruitment and UCC after DNA damage occurred in some but not all p53-compromised cells. Exponentially growing cells were treated with 200 ng/ml NCS and, 24 h, were later processed for immunostaining. SMC2, red; DNA (DAPI), blue. Bars, 20 µm. (C) MCD developed in cells that exhibited hallmarks of premature mitosis. Cells were treated with 200 ng/ml NCS for 72 h, stained with Hemacolor reagents, and photographed under light microscopy. M-labeled arrows indicate MCD; A-labeled arrows point to apoptotic cells. All samples were viewed under identical microscope settings.