Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200609174
Suzuki et al.

• Video 1 Representative movement of a CD59 cluster (IgG-gold particle) recorded at video rate (replayed in real time), showing alternating periods of apparently simple Brownian diffusion and STALL.

• Video 2 Single-molecule detection of transient Lyn-GFP recruitment to a CD59 cluster (occurring constitutively) without temporal correlation with the STALL of the CD59 cluster.

• Video 3 Single-molecule detection of transient Gαi2(YFP) recruitment to a CD59 cluster, which occurs right before the onset of the STALL of the CD59 cluster.

• Video 4 Single-molecule tracking of Gαi2(YFP), showing that Gαi2(YFP) is recruited from the cytoplasm to the plasma membrane only transiently, i.e., its residency time on the membrane is on the order of 0.1 s.

• Supplemental text (PDF)