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Figure S2. Flag-AGO2 colocalized and associated with endogenous Drosophila GW in S2 cells. (A and B) Flag-AGO2 in S2 cells fixed 80 min after induction by heat shock from the hsp70 promoter colocalizes with endogenous GW. (C) Extracts of S2 cells either transfected with Flag-AGO2 (+) or untransfected (-) were immunoprecipitated with anti-GW and Western blotted using anti-Flag antibody. Arrowhead points to the band representing FLAG-Ago. Bars, 20 µm.