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Video 2
A time-course video showing an abnormal pattern of chromosome division in a homozygous gw¹ mutant expressing histone-GFP. The video begins at ∼30 min AED. By 3,000 s, nuclei begin to appear at the embryo periphery. However, several differences are seen compared with wild-type embryos, as there is a lack of cytoplasmic clearing in the majority of the embryo, and fewer than normal nuclei are seen at the cortex at the anterior of the embryo compared with the posterior. In subsequent NCs, the chromosomes within the remaining cortical nuclei fail to undergo mitosis successfully, partially separating but then rejoining. Several “paired” structures were seen that resolved into larger than normal interphase nuclei. After one more NC, very few nuclei are seen that resemble wild type, and the remaining chromatin moves freely within the cytoplasm and begins to fuse into large aggregates comparable with what is observed with DNA staining (Fig. 2 B). Images were captured at 1 frame/10 s and are displayed at 20 frames/s (∼200x speed).