Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation -- Data Supplement - JCB--Montpetit et al. -- The Journal of Cell Biology

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Figure S1. ndc10-4xK→R strains do not show defects related to cytokinesis. (A) Septin ring formation is normal in ndc10-4xK→R strains. Cdc10-GFP was used as a marker for the septin ring in WT and ndc10-4xK→R strains, and, unlike ndc10-1 (Bouck, D.C., and K.S. Bloom. 2005. Proc. Natl. Acad. Sci. USA. 102:5408-5413), septin rings mature and split normally. (B) Haploid axial bud pattern is unaffected in ndc10-4xK→R mutants. WT and ndc10-4xK→R strains were grown at 30°C and fixed. Bud scars were stained with Calcofluor white. Bars, 5 µm.