Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200604014
Wu et al.

• Figure S1 STIM1 accumulation precedes I_CRAC activation during IP₃-induced Ca²⁺ release.

• Figure S2 Fluorescence-quenching measurements using wide-field epifluorescence microscopy to assay for plasma membrane-localized GFP-STIM1.

• Figure S3 Immunofluorescence staining of HA-tagged STIM1 in Jurkat cells before and after store depletion.

• Video 1 GFP-STIM1 redistribution in an intact cell after store depletion with TG.

• Video 2 STIM1 accumulation near the plasma membrane during I_CRAC activation.

• Video 3 Assaying for plasma membrane-localized GFP-STIM1 puncta by fluorescence-quenching and TIRF microscopy.

• Video 4 Assaying for plasma membrane-localized GFP-STIM1 puncta by fluorescence-quenching and wide-field epifluorescence microscopy.