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Figure S1. GFP-CLIP-170 localization in WT or TTL-null fibroblasts. (A) EB1 and GFP-CLIP-170 localization in WT or TTL-null (TTL KO) fibroblasts. Fibroblasts were transfected with GFP-CLIP-170 and EB1 and double stained with EB1 and GFP antibodies. In WT cells and in Tyr+ TTL-null cells, GFP-CLIP-170 and EB1 colocalized at most MT tips, whereas GFP-CLIP-170 comets were lacking in Tyr- TTL-null cells, which contained large aggregates of mislocalized GFP-CLIP-170. Note that the presence of GFP-CLIP-170 aggregates did not preclude GFP-CLIP-170 association with MT ends in WT cells. Bar, 10 µm. (B) Quantitative analysis of GFP-CLIP-170 localization in WT or TTL-null fibroblasts. MT plus ends were identified by EB1+ signal, and the percentage of GFP-CLIP-170+ ends within EB1 MT ends was quantified (% CLIP-170/EB1). Mean values ± SEM of percentage of CLIP-170/EB1 in WT (n = 36), TTL-null Tyr+ (n = 71), and TTL-null Tyr- cells (n = 44). ***, P t test).