Video 8
The depletion of Spindly, dynein, or Rod slows the alignment of chromosomes on the metaphase plate. Cells stably expressing GFP-H2B (red) and mCherry-α-tubulin (green) were treated with the indicated dsRNAs, plated in 96-well plates, and then fields of cells were imaged every minute for 2 h using an automated microscope. Cells that entered mitosis with clearly separated centrosomes were then selected from each field and isolated, and image stacks were converted to videos. Field of view is 24 µm.