[View Larger Version of this Image]

Figure S5. The depletion of NP_060255 causes defects in chromosome alignment. (a) Potential Spindly homologues were identified in multiple higher eukaryotic species. The alignment shows the region of highest homology between all of the proteins (the numbers next to the species name indicate the amino acids that are shown); darker colors indicate conserved residues. (b and c) Control (b) and NP_060255 (c) siRNA-treated cells were imaged using an automated microscope, and mitotic cells were identified. Typical metaphase images are shown for the indicated conditions. Arrows indicate misaligned chromosomes. Bar, 10 µm.