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Figure S2. YPP1 fails to prevent ROS generation by H₂O₂. Fluorescence microscopy in conjunction with the DHR 123 dye was used to determine ROS accumulation in FY23 cells transformed with the pTF602 (YPP1) plasmid or pTF604 (empty vector). Experiments were performed after 3 h in inducing media. (A) Induced cells were incubated with 3 mM H₂O₂, washed, stained with DHR 123, and then visualized by fluorescence microscopy. (B) Plot of ROS % (mean ± SD). The number of cells counted in each group were n = 1154 to 2089. *, P = 0.0016. **, P = 0.00026.