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Video 1
A time-lapse video of a BMM transduced with Syt VII-YFP and exposed to IgG-opsonized zymosan at a particle/cell ratio of 10. Syt VII-YFP, green. 5 frames/s. Differential interference contrast (bottom) and YFP (top) were imaged simultaneously every 18 s. At the top right, Syt VII recruitment to a phagocytic cup can be clearly seen. At the bottom left, two additional particles can be followed as they enter the BMM. Syt VII recruitment to areas of intense plasma membrane ruffling can also be observed at the late stages of particle uptake and in the final frames of the video. Supporting information for Fig. 5.