Video 2
Transitions between closed-angle and open-angle conformations of L-selectin. Dramatically different dynamics were observed in this simulation, which was run under identical conditions to Video 1. At the beginning, the EGF domain of the simulated L-selectin structure matched well with the crystal structure of closed-angle L-selectin because the simulation began with that structure. Over the 6-ns simulation time, the simulated L-selectin structure was observed to change its conformation by straightening the interdomain hinge to an open angle, allowing the EGF domain to match that of open-angle P-selectin. The video was generated with VMD software (Molecular Graphics; Humphrey et al., 1996.)