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Video 4
IL-6 exiting the Golgi in a budded vesicle and fusing with the recycling endosome. Live epifluorescence imaging of a macrophage transfected with IL-6-GFP. Section of a cell extracted in the video as demonstrated in Fig. 5 C. Video is shown at 10 frames per second, with individual frames captured at 2-s intervals, and an intensity-based color display filter was used to emphasize structures. The video demonstrates multiple trafficking events, but attention is drawn to the exit of vesicles from the intensely stained Golgi region and fusion of some of these vesicles with preexisting structures within the cytoplasm of the cell.