Video 6
IL-6 does not traffic to the forming phagocytic cup. Live epifluorescence imaging of a macrophage dual transfected with VAMP3-GFP (green) and IL-6-mCherry (red) during phagocytosis of IgG-opsonized 3-µm latex beads. Delays between capturing separate fluorophores in dual-transfected cells was 81 ms, and capture rates of fluorophores was 200 (mCherry) and 500 ms (GFP). Video playback is shown at 10 frames per second. Attention is drawn to the lack of IL-6-mCherry traffic toward the forming phagocytic cup despite the large-scale delivery of VAMP3.