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Figure S3. Glutamine deprivation reduces production of lactate. IER-MYC-C9DN cells were treated with OHT or ethanol for 24 h in 12-well plates, Cells were washed once with PBS, and 1 ml of media with or without glutamine was added. At the indicated time points, the media was collected. Amount of lactate in 1 milliliter of media in each well is plotted against the time of incubation in the presence or absence of glutamine. Results of the first experiment are presented as an average of three replicate samples. Error bars indicate SD. Results of the second experiment are presented as an average of two replicate samples.