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Figure S1. PCR analysis of BMPRII expression and Western blotting of ADF/cofilin in Xenopus neural tube tissues. (A) Expression of the long form of BMPRII in Xenopus neural tube tissues. RT-PCR using two different primer sets on purified Xenopus neural tubes detects only the long form of BMPRII. Sequencing of the PCR products confirms that they are the long form of Xenopus BMPRII. (B) Western blot analysis using XAC and p-XAC antibodies detects a predominant band in 2 µg of Xenopus whole embryo cell lysates.