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Figure S3. Examination of the degree of colocalization between the multiple Nups that mislocalize to cytoplasmic foci in the absence of Apq12. Colocalization analysis of Nup159/Rat7 and Nup82-GFP (using IF), Nup159/Rat7 and Nup170-GFP (using IF), and Nup82-GFP and Nup188-RFP (live cell microscopy) in WT and apq12Δ cells at 23°C.