Dynamic subcompartmentalization of the mitochondrial inner membrane -- Data Supplement - JCB--Vogel et al. -- The Journal of Cell Biology

Table S1. Quantitative immuno-EM of mitochondrial proteins

A. Subcompartmental location of mitochondrial proteins in wild type during exponential growth phase on YPLac
Protein	# gold OM/IBM	# gold CM	# gold back-ground	# total gold	Percentage of gold particles per unit OM/IBM membrane length	Percentage of gold particles per unit CM membrane length	# of experiments/ remarks
					%	%
Aac2	66	124	11	201	43.5	56.5
Core1	87	250	20	357	32.9	67.1
Core2	66	190	10	266	33.4	66.6
Cox2	75	135	10	220	44.8	55.2	#1^a
Cox2	66	120	13	199	44.2	55.8	#2^a
Cox2	39	110	13	162	32.7	67.3	#3^a
Cox2	180	369	32	581	40.6 ± 6.8	59.4 ± 6.8	#1–3
Isd11	65	126	9	200	42.9	57.1
Mgm1	160	152	5	317	61.2	38.8
Mia40	92	109	13	214	55.5	44.5
Mrpl36	58	117	21	196	40.6	59.4
Nfs1	51	135	18	204	34.0	66.0
Oxa1	53	108	13	174	41.1	58.9
Phb1	65	126	19	210	42.0	58.0
Phb2	89	138	23	250	48.0	52.0
Rip	130	224	22	376	45.7	54.3
Su e	126	226	23	375	44.6	55.4
Su g	96	202	29	327	39.9	60.1
Tim14	471	351	53	875	66.9	33.1
Tim16	436	365	46	847	64.2	35.8
Tim17	103	105	9	217	59.4	40.6
Tim23	81	95	4	180	56.0	44.0	#1^a
Tim23	144	141	9	294	60.4	39.6	#2^a
Tim23	131	119	9	259	62.2	37.8	#3^a
Tim23	147	130	18	295	62.8	37.2	#4^b
Tim23	122	88	16	226	67.7	32.3	#5^b
Tim23	356	357	20	733	59.5 ± 3.2	40.5 ± 3.2	#1–3^a
Tim23	625	576	53	1254	63.4 ± 3.9	36.6 ± 3.9	#1–3/#4/#5^b
GFP- Tim23	140	38	36	214	87.4	12.6	#6^c
Tim50	112	114	14	240	59.4	40.6	#1^a
Tim50	168	98	14	280	72.2	27.8	#2^a
Tim50	184	138	11	333	66.7	33.3	#3^a
Tim50	464	350	39	853	66.1 ± 6.5	33.9 ± 6.5	#1–3^a
Tom20	139	43	31	213	84.9	15.1
Tom40	113	52	14	179	77.2	22.8
B. Dynamic redistribution of Tim23 and Mrpl36 in wild type overexpressing mtGFP during exponential growth on YPLac
GFP	39	55	162	256	–	–	no puromycin treatment
Tim23	104	89	4	197	63.7	36.3	no puromycin treatment #1
Tim23	211	142	13	366	69.1	30.9	no puromycin treatment #2
Tim23	221	150	7	378	68.9	31.1	no puromycin treatment #3
Tim23	536	381	24	941	67.2 ± 3.1	32.8 ± 3.1	no puromycin treatment #1/#2/#3
Tim23	68	102	10	180	49.4	50.6	30 min puromycin treatment#1
Tim23	107	134	7	248	54.3	45.7	30 min puromycin treatment #2
Tim23	104	135	7	246	53.4	46.6	30 min puromycin treatment #3
Tim23	279	371	24	674	52.4 ± 2.6	47.6 ± 2.6	30 min puromycin treatment #1-3
Mrpl36	56	118	16	190	40.0	60.0	no puromycin treatment
Mrpl36	39	69	63	171	–	–	30 min puromycin treatment
C. Subcompartmental location of complex III and IV subunits in the wild type and in the Δbcs1 strain during exponential growth on YPGal
Cox2	89	106	10	205	45.3	54.7	wild type
Cox2	50	89	14	153	34.7	65.3	Δbcs1
Core1	85	191	16	292	29.9	70.1	wild type
Core1	93	136	8	237	40.3	59.7	Δbcs1
Rip	79	124	11	214	38.3	61.7	wild type
Rip	92	102	8	202	47.2	52.8	Δbcs1

The number of gold particles after immunogold labeling with antibodies raised against the indicated protein for each of the three analyzed subcompartments (OM /IBM, CM, and background) are given. The percentage of gold per OM/IBM or CM membrane length is calculated by first subtracting the normalized background determined for each experiment and then by normalizing for membrane length (see Materials and methods).

^aIdentically fixed cells.

^bSeparately grown and processed cells.

^c Anti-GFP antibody.