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Figure S1. Effects of RNAi against msk and ketel on cNLS-driven nuclear import. (A) S2 cells were treated with indicated RNAi. After Dpp stimulation, whole-cell extracts were prepared and analyzed by immunoblotting with the indicated antibodies. (B) Expression vector encoding two copies of GFP fused to a cNLS (cNLS-GFP) was transfected into Drosophila S2R+ cells. After indicated RNAi, cNLS-GFP was expressed by CuSO4 induction. In cells with control or msk RNAi, the GFP signal was predominantly in the nucleus, whereas in cells with ketel RNAi the GFP signal was more diffusive throughout the cell. Ketel knockdown also changed the morphology of the S2R+ cells. Bar, 10 µm.