Parkin-mediated K63-linked polyubiquitination targets misfolded DJ-1 to aggresomes via binding to HDAC6 -- Data Supplement - JCB--Olzmann et al. -- The Journal of Cell Biology

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Figure S3. High levels of parkin overexpression have no effect on the degradation of L166P mutant DJ-1. SH-SY5Y cells were transfected with the indicated ratios of HA-tagged wild-type or L166P mutant DJ-1 and Myc-tagged parkin. Lysates were resolved by SDS-PAGE and analyzed by Western blotting.