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Figure S4. The misfolded L166P mutant DJ-1 colocalizes with ubiquitin and is concentrated in the center of the aggresome. SH-SY5Y cells coexpressing GFP-tagged parkin and HA-tagged L166P mutant DJ-1 were incubated in the presence of 20 µM MG132 for 16 h, fixed, and immunostained as with antibodies against HA and ubiquitin or vimentin as indicated. Z-sections were taken at an interval of 1 µm with a Zeiss confocal microscope. The colocalization between parkin and L166P mutant DJ-1 is indicated by the yellow color. The colocalization of parkin, L166P mutant DJ-1, and ubiquitin is indicated by the white color. Bar = 10 µm.