Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200706012
Yam et al.

• Figure S1 Automated cell outline determination.

• Figure S2 The prospective rear edge thickness decreases early during motility initiation.

• Figure S3 Formation of an actin axle at the cell rear occurs during maturation of the polarized form.

• Figure S4 Perturbation of myosin II activity does not perturb motile cell polarity.

• Figure S5 Removal of stationary background features.

• Video 1 Visualization of F-actin network movement in stationary keratocytes with FSM.

• Video 2 Visualization of F-actin network movement in motile keratocytes with FSM.

• Video 3 Spontaneous symmetry breaking and motility initiation of keratocytes.

• Video 4 Spontaneous symmetry breaking and motility initiation of keratocytes.

• Video 5 Radially symmetric F-actin flow field in a stationary keratocyte.

• Video 6 Increase in rear F-actin flow speed and a reorientation of the perinuclear F-actin network flow in a keratocyte initiating motility.

• Video 7 Local application of calyculin A can stimulate motility initiation.

• Video 8 Formation of an actin axle during the maturation of polarity.