Table S1. Yeast strains used in this study

Name

Genotype

Source

DDY426

MATa/α, his3-Δ200/his3-Δ200 leu2-3, 112/leu2-3, 112 ura3-52/ura3-52 ade2-1
ADE2 lys2-801/lys2-801

Drubin/Barnes laboratory

DDY427

MATa/α, his3-Δ200/his3-Δ200 leu2-3, 112/leu2-3, 112 ura3-52/ura3-52 ade2-1/ADE2
lys2-801/lys2-801 cof1-Δ1::HIS3/COF1

Drubin/Barnes laboratory,
Moon et al., 1993

DDY1102

MATa/α, his3-Δ200/his3-Δ200 leu2-3, 112/leu2-3, 112 ura3-52/ura3-52 ade2-1/ADE2
lys2-801/LYS2

Drubin/Barnes laboratory

DDY3444a

MATα, his3-Δ200, leu2-3, 112, ura3-52, ADE2, lys2-801, cof1-Δ1::HIS3
ABP1-mRFP::HIS3 +pDD2090

This study

DDY3445a

MATa, his3-Δ200, leu2-3, 112, ura3-52, ADE2, lys2-801, ABP1-mRFP::HIS3
+pDD2091

This study

DDY3446b

MATα, his3-Δ200, leu2-3, 112, ura3-52, LYS2, sla2Δ::cgLEU2c ABP1-mRFP::HIS3
+pDD302

This study

DDY3447b

MATα, his3-Δ200, leu2-3, 112, ura3-52, LYS2, sla2Δ::cgLEU2c ABP1-mRFP::HIS3
+pDD2091

This study

DDY3448b

MATα, act1-159::HIS3 his3-Δ200, leu2-3, 112, ura3-52, LYS2, sla2Δ::cgLEU2c
ABP1-mRFP::HIS3 +pDD2091

This study

DDY3449a

MATα, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, sla2Δ::cgLEU2c COF1::LEU2
+pDD302

This study

DDY3450a

MATa, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, sla2Δ::cgLEU2c cof1-22::LEU2
+pDD302

This study

DDY3451a

MATα, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, Sla1-GFP::KanMX6
Abp1-mRFP::HIS3

This study

DDY3452a

MATα, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, Sla1-GFP::KanMX6
Abp1-mRFP::HIS3 cof1-22::LEU2

This study

DDY3453a

MATa, his3-Δ200, leu2-3, 112, ura3-52, ADE2, lys2-801, ABP1-GFP::KanMX6

This study

DDY3454a

MATa, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, bar1Δ::HIS3 COF1::LEU2

This study

DDY3455a

MATa, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, bar1Δ::HIS3 cof1-22::LEU2

This study

DDY3456a

MATa, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, Abp1-GFP::KanMX6 cof1-22::LEU2

This study

DDY1252a

MATα, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, COF1::LEU2

Drubin/Barnes laboratory,
Lappalainen and Drubin, 1997

DDY1266a

MATα, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, cof1-22::LEU2

Drubin/Barnes laboratory,
Lappalainen and Drubin, 1997

DDY3459a

MATa, his3-Δ200, leu2-3, 112, ura3-52, lys2-801, sla2Δ::cgLEU2c, Abp1-mRFP::HIS3,
pdr5Δ::KanMX6, snq2Δ::HIS3 +pDD2091

This study

aStrains derived from DDY426.

bStrains derived from DDY1102.

References

Lappalainen, P., and D.G. Drubin. 1997. Cofilin promotes rapid actin filament turnover in vivo. Nature. 388:78–82.

Moon, A.L., P.A. Janmey, K.A. Louie, and D.G. Drubin. 1993. Cofilin is an essential component of the yeast cortical cytoskeleton. J. Cell Biol. 120:421–435.