The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways -- Data Supplement - JCB--Alto et al. -- The Journal of Cell Biology

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Figure S2. (A) Wide-field fluorescence microscopy images depicting EGFP-EspF and Rhodamine-phalloidin (actin) in SNX9-transfected HeLa cells. (B) Wide-field fluorescence microscopy images depicting mCherry-SNX9 and FITC-phalloidin (actin) [top images] or microtubules (anti-tubulin) [bottom images] in EspF-transfected HeLa cells. (C) Quantification of the percentage of cells forming new membrane tubules in EspF/SNX9 expressing HeLa cells. Cells were treated with vehicle (DMSO) (white bar) or 1 µM Latrunculin-A (black bar) for 1 h before fixation. (D) Quantification of the percentage of cells in which membrane tubules co-localize with F-actin filaments in EspF/SNX9-expressing HeLa cells. Cells were treated with vehicle (DMSO) (white bar) or 1 µM Latrunculin-A (black bar) for 1 h before fixation.