Requirement of biphasic calcium release from the endoplasmic reticulum for Fas-mediated apoptosis -- Data Supplement - JCB--Wozniak et al. -- The Journal of Cell Biology

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Figure S1. FasL induces rapid and long-term elevations in mitochondrial calcium. (A) Pseudocolor 495:380 ratio images of a single Jurkat cell expressing mt-ratiometric pericam. Treatment with FasL induces rapid increases in calcium in all mitochondria. Some mitochondria have persistently elevated calcium (white arrowhead), whereas others have transient and/or oscillatory increases in calcium (red arrowhead). (B) Quantification of increases in global mitochondrial calcium levels in a single Jurkat cell. (C) Mitochondrial calcium in a cell pretreated with 400 nM B-IP3RCYT. (D) Average mitochondrial steady-state calcium levels after 24 h FasL treatment. Bars represent the mean ± the SEM of 30 cells.