An FHA domain-mediated protein interaction network of Rad53 reveals its role in polarized cell growth -- Data Supplement - JCB--Smolka et al. -- The Journal of Cell Biology

Table S2. Plasmids used in this study

Plasmid	Description	Source
pMBS1^a	PATH-FHA1	This study
pMBS2^a	PATH-FHA1 R70A	This study
pMBS3^a	PATH-FHA2	This study
pMBS4^a	PATH-FHA2 R605A	This study
pMBS5^b	GST-FHA1	This study
pMBS6^b	GST-FHA1 R70A	This study
pMBS7^b	GST-FHA2	This study
pMBS8^b	GST-FHA2 R605A	This study
pMBS9^c	Xpress-FHA1	This study
pMBS10^c	Xpress-FHA1 R70A	This study
pMBS11^c	Xpress-FHA2	This study
pMBS12^c	Xpress-FHA2 R605A	This study
pMVB24	GST-CDC10	D. Thorner (University of California, Berkeley, Berkeley, CA)
pGST-CDC11	GST-CDC11	D. Kellogg (University of California, Santa Cruz, Santa Cruz, CA)
pMVB25	GST-CDC12	D. Thorner
pMVB27	GST-CDC3	D. Thorner
PBEG4^b	GST-SHS1	D. Thorner
pMBS15^d	6HIS-Rad53	Smolka et al., 2005
^aThese vectors are based on pET21a (Novagen). PATH tag was inserted using NdeI and BamHI. FHA domains were inserted between EcoRI and NotI cloning sites. ^bFHA domains were cloned into pGEX4T1 (GE Healthcare) using EcoRI and NotI cloning sites. ^cFHA domains were cloned into pYES2/NT c (Invitrogen). ^dVector is pET28a.