Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane -- Data Supplement - JCB--Witzel et al. -- The Journal of Cell Biology

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Figure S3. FGF8 receptor distribution at sites of Wnt11-induced Fz7 accumulations. One-cell-stage embryos were injected with 90 pg fgf8-receptor-rfp (fgf8r-rfp), 60 pg fz7-yfp, and 20 pg wnt11 mRNA. Blastodermal cells of fixed pregastrula-stage embryos (30% epiboly; 5 hpf) were imaged by confocal microscopy at the animal pole. (A-C) Blastodermal cells expressing Fz7-YFP (A) and FGF8R-RFP (B) in the presence of exogenous Wnt11. A merged image is presented in C. Arrowheads point out sites of Wnt11-induced Fz7-YFP accumulations at the plasma membrane. Bar, 10 µm.