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Video 1
Separating cells in pregastrula-stage embryos expressing Fz7-YFP and Wnt11. Embryos were injected at the one-cell stage with 60 pg of fz7-yfp and 10 pg of wnt11 mRNA. 3D time-lapse analysis was performed by two-photon microscopy of blastodermal cells at the animal pole of pregastrula-stage embryos (30% epiboly; 5 hpf). The video corresponds to the image sequence in Fig. 5 A and shows the separation behavior of two cells exhibiting Wnt11-induced Fz7-YFP accumulation at their sites of cell contact. Arrowheads indicate sites of cell contacts. Time intervals, 130 s. Bar, 10 µm.