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Figure S2. Transport efficiency dependence on energy source. These anti-SufI immunoblots show pre-SufI transport assays performed under standard conditions (see Fig. 1 C) when IMVs were energized by different concentrations of succinate (black), ascorbate and 0.3 mM PMS (red), and NADH (blue). For this figure only, the Buffer B (see Materials and methods) used to make IMVs did not contain KCl. Under the tested conditions, energization with 4 mM NADH resulted in the best SufI transport efficiencies (72%).