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Figure S3. Midgut constriction and muscle attachment phenotypes in embryos lacking mib2 activity. Shown are stage 16 embryos with mib2⁴ (A), mib2¹ (C), and wild-type (B and D) chromosomes in trans to Df(2L)TW130 and derived from homozygous mutant germ lines. (A) Tropomyosin-stained mib2 mutant embryo with unconstricted midgut. (B) Control embryo stained as in panel A with midgut constrictions. (C) Tropomyosin (red) and anti-phospho-FAK–stained (green) mib2 mutant embryo, showing phospho-FAK localized at normal muscle attachment sites and at sites of muscles undergoing detachment and degeneration. (D) Control embryo stained as in panel B showing phospho-FAK localization (green) to muscle attachments.