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Figure S2. Localization of appendage proteins during the cell cycle. Asynchronously growing U2OS cells were analyzed for the localization of appendage proteins during interphase and various mitotic stages, respectively. (A) Colocalization of Cep164 (green) and γ-tubulin (red). Note that Cep164 persists at centrioles throughout M phase. (B) Colocalization of Cep164 (green) and ninein (red). Note that ninein is displaced from centrioles during M phase. (C) Colocalization of Cep164 (green) with Cep170 (red). Note that Cep170 is displaced from centrioles during M phase but associates with the mitotic spindle (Guarguaglini, G., P.I. Duncan, Y.D. Stierhof, T. Holmstrom, S. Duensing, and E.A. Nigg. 2005. Mol. Biol. Cell. 16:1095–1107). Bars, 10 µm.