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Video 10
Time-lapse fluorescence of YFP-actin (left, green) and Alexa546-actin (middle, red) in the MCF-7 cells coexpressing YFP-actin and control siRNA. Immediately after microinjection of Alexa546-actin monomers, the cells were stimulated with NRG (A; shown in Fig. 8 D) or left unstimulated (B; shown in Fig. S4 D) at time 0. Images were acquired every 30 s for 9.5 min and are displayed at 7 frames/s. Merged images are shown at the right. Bars, 20 µm.