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Video 2
Time-lapse fluorescence of Dp-actin in the COS7 cells shown in Fig. 1, B and C, and Fig. 2, A and B. COS7 cells expressing Dp-actin were untreated (A) or treated with Jasp (B) before photoactivation. (C and D), COS7 cells were cotransfected with Dp-actin and LIMK1(WT) (C) or LIMK1(D460A) (D). Images were acquired every 0.4 s for 7.2 s and are displayed at 7 frames/s. Bars, 20 µm.