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Figure S3. (A) Wild-type and vsk3 mutant strains have similar rates of pinocytosis. Cells were incubated with TRITC-dextran at room temperature. Samples were collected and quenched with Trypan blue, and intracellular TRITC fluorescence was measured for equivalent cell numbers. (B) Fluorometric analysis of phagocytosis of WT and vsk32 cells is compared with WT and vsk32 cells overexpressing VSK3ΔK-YFP (VSK3ΔK-YFP/WT or VSK3ΔK/vsk32, respectively). (C) FACS analysis of phagocytosis of VSK3-K518R-YFP/WT, vsk32, and VSK3-K518R-YFP/vsk32 is shown.