Movie 1. Steady-state experiment. Time-lapse of product formation in microchambers that contain a single enzyme molecule. By following the substrate turnover trajectories of single enzyme molecules, the independent signal increase and decrease due to photobleaching is evident. The trajectories reflect the stochastic events of repeated inhibitor binding and release.

Movie 2. Pre-steady-state experiment. Time-lapse of product formation in microchambers that contain a single enzyme molecule. Three images of this movie are shown in Fig. 2A. By following the substrate turnover trajectories of single enzyme molecules, the delayed onset of substrate turnover due to a stochastic event of inhibitor release is evident.