Fig. 5. Fat volume, as a function of body mass, for both the CTR (Left) and LMMS (Right) B6 mice. Whereas CTR animals showed a strong positive correlation between fat volume and weight (r² = 0.70; P = 0.0001), correlation in the LMMS animals was weak (r² = 0.18; P = 0.1). A comparison between slope and intercept shows the control and LMMS to be significantly different (P < 0.001). Considered with the similar food intake between groups, these data indicate that LMMS suppressed adiposity. (n = 15 in each group).

Fig. 6. Automated gating of flow cytometry data ensured that the same cell population was analyzed in each sample harvested from the marrow and adipose tissue in the GFP⁺ recipients. At 6 weeks, representative data for a bone marrow sample (A) identifies the two principal regions used in the analysis, R1 and R2. Phycoerythrin-conjugated antibodies against an isotype control demonstrated a low level of nonspecific binding (B), but phycoerythrin-conjugated anti-Sca-1 to specifically label MSCs shows a preferential localization to R1 (C). FSC-H, forward scatter; SSC-H, side scatter. Also shown is the 19% reduction of GFP⁺ adipocytes to GFP⁺ MSCs (Lower Left), with 12% less weight of the epididymal fat pad of LMMS animals (Lower Right). *, P < 0.05.

Fig. 7. Time course for the overall experimental design, indicating the period over which mice were subjected to LMMS. Specifics regarding the mouse model, number in each group, and age at baseline and killing are also given. Although in vivo scans were performed at 12 weeks in experiment 1 and at 9 weeks in experiment 3, the percentage of adipose tissue suppressed by LMMS relative to control was similar.