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Video 5
Cofilin siRNA knockdown suppresses EGF-induced F-actin assembly and protrusion in MTLn3 cells. MTLn3 cells were treated with the cofilin siRNA for 4 h. 2 h after termination of the transfection, they were plated^{on glass bottom dishes. Around 18 h after the cells have adhered, they were starved for 3–4 h, and then EGF (5 nM) was added to the media (bath application) and cells were imaged for 10 min, after addition of EGF, at a rate of 6 frames/min. At the time of imaging, cells were at 36 h after the siRNA treatment.}