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Video 3
Expression of the WT(ΔH) cofilin plasmid rescues the directional walking behavior characteristic of the cofilin siRNA knockdown cells. MTLn3 cells were treated with the cofilin siRNA for 4 h. 2 h after termination of the transfection, they were plated^{on glass-bottom dishes. When cells attached (~ 8 h), they were transfected with the WT(ΔH)cofilin plasmid for 45 min. After 21 h (total 36 h after siRNA treatment), cells were imaged for 45 min, at a rate of 2 frames/min, in L15-5% FBS-contained media overlaid with mineral oil, on a 37°C stage.}