Fig. 9. Physical and transcriptional map of the murine bcsc-1 gene. (A Upper) Genomic and transcriptional organization of the murine BCSC-1 gene locus. The complete genomic structure of the murine BCSC-1 gene is contained within the complete sequence of BAC clone AC069561. The gene comprises 20 exons and spans 25 kb. The position of a consensus sequence generated by direct sequencing of PCR amplifications of BAC clone 271c5, using primers between exons 3 through 11, is indicated. The relative positions of contiguous cDNA sequences derived from sequencing of image clones and RACE product combined with representative mouse ESTs from UNIGENE cluster Mm.34060 (Note: only 13 overlapping, non-redundant mouse ESTs were used for clarity.) The coding exon regions are highlighted in red whereas conserved domains identified in the Conserved Domain Database (www.ncbi.nlm.nih.gov/Structure/cdd/cdd.shtml) and their relative positions are in gray. (Lower) Protein comparison of the human and murine BCSC-1 proteins. Pairwise analysis of the human and mouse BCSC-1 proteins was performed using the blastp program (www.ncbi.nlm.nih.gov/). The results indicate a 67% identity and a 78% similarity between proteins. Boxed letters indicate identical amino acid sequences. (B) Northern blot analysis of bcsc-1 gene expression. A mouse Multiple Tissue Blot (BD Clontech) was hybridized with a PCR-synthesized cDNA probe containing the complete coding sequence. b -actin was used as a control.