Fig. 11. Ectopic BCSC-1 expression by lentiviral-mediated gene transfer of the lung adenocarcinoma cell line A549. Flow cytometric and cell cycle analysis of cells uninfected or infected with pNaldini.CMV.IRES.EGFP or pNaldini.CMV.BCSCHA.IRES.EGFP viruses pseudotyped with the VSV glycoprotein. GFP expression was analyzed in live cells, and DNA content was determined by propidium iodide staining 24 h and 48 h postinfection. Shown is expression analysis of A549 cells using the anti-HA (HA.11; Covance) and anti-GFP (Roche) antibodies by Western blot analysis.