Table 2. Primers used for PCR to generate vectors expressing the E2p7NS2 genes of HCV

1431F+KpnI

5'-CGGGGTACCATGGTGGGGAACTGGGCGAAGGTCCTGG-3'

3419Rter+EcoRI

5'-CCGGAATTCTTACTACAGCAACCTCCACCCCTTGGAGACCAT-3'

1900F

5'-ACAGGTCGGGCGCGCCTACCTACAGCTGGG-3'

2579R+15

5'-CACCTCCGTGTCCAGCGCCTCCGCTTGGGATATGAGTAAC-3'

2769F+15

5'-TCCCAAGCGGAGGCGCTGGACACGGAGGTGGCCGCGTCGT-3'

2989R

5'-GTCGGGTGTACTACACACATGAGTAAGATG-3'

2624R+15

5'-GAGCAGGAGGAGAGGCCCGGCCAGGGATGCTGCATTGAG-3'

2724F+15

5'-GCATCCCTGGCCGGGCCTCTCCTCCTGCTCCTGCTGGCGTTG-3'

2693R(M)

5'-GGGCACCCAGCTACCTATCAGATACCACGCAAAGCA-3'

2667F(M)

5'-TGGTATCTGATAGGTAGCTGGGTGCCCGGAGCGGTC-3'

E2ter+15

5'-GGAATTAATTCGCTACGCCTCCGCTTGGGATATGAG-3'

terIRES+15

5'-CAAGCGGAGGCGTAGCGAATTAATTCCGGTTATTTT-3'

IRES-R

5'-TGGCCATGGTATTATCATCGTGTT-3'

Sec-F

5'-AATGGGAGTTTGTTTTGGCAC-3'

Sec-R+15

5'-GAGGTTCTCCAAAGCGTCACCAGTGGAACCTGGAAC-3'

p7NS2+15

5'-GGTTCCACTGGTGACGCTTTGGAGAACCTCGTAATA-3'

p7-R+IRES

5'-ACCGGAATTAATTCGCTATGCGTATGCCCGCTGAGGCAA-3'

NS2-F+sig

5'-GGTTCCACTGGTGACCTGGACACGGAGGTGGCCGCG-3'

IRES-F+p7

5'-CAGCGGGCATACGCATAGCGAATTAATTCCGGTTATTTT-3'

sig-R+NS2

5'-CACCTCCGTGTCCAGGTCACCAGTGGAACCTGGAAC-3'

E2R-J6p7

5'-CAGCTTCTCTAGTGCCGCCTCCGCTTGGGATATGAG-3'

NS2F-J6p7

5'-CAACAGGCTTATGCTCTGGACACGGAGGTGGCCGCG-3'

J6p7-F

5'-TCCCAAGCGGAGGCGGCACTAGAGAAGCTGGTCATC-3'

J6p7-R

5'-CACCTCCGTGTCCAGAGCATAAGCCTGTTGGGGCAA-3'

N-tail-R

5'-ATTGCAGCTAGCTGCGGATGCTGCATTGAGTATTAC-3'

C-tail-F

5'-CTCCTAGCATTGCCACAGCGGGCATACGCACTGGAC-3'

N-tail-F

5'-CTCAATGCAGCATCCGCAGCTAGCTGCAATGGCTTC-3'

C-tail-R

5'-TGCGTATGCCCGCTGTGGCAATGCTAGGAGCAGTAG-3'

cyto-R

5'-TCCGGGCACCCACCTACCCTTCAGGTACCAAGCAGCCACGAAAAA-3'

cyto-F

5'-CTGAAGGGTAGGTGGGTGCCCGGAGCTACCTATTCCCTCACTGGC-3'

TMD-1R

5'-CGCAGCGTGCAAGATGACCAGCTTCTCTAGTGCCGCCTCCGCTTGGGATATGAG-3'

TMD-2F

5'-CTAGAGAAGCTGGTCATCTTGCACGCTGCGAGCCTGGCCGGGACGCACGGTCTT-3'

TMD-2R

5'-TAAGGGGACTACCCGACCCTTGATATACCACGCAAAGCAGAAGAA-3'

TMD-3F

5'-ATCAAGGGTCGGGTAGTCCCCTTAGCGGTCTACGCCCTCTACGGG-3'

TMD-3R

5'-CACCTCCGTGTCCAGAGCATAAGCCTGTTGAGGCAACGCCAGCAGGAGCAG-3'