Table 2. Primers used for PCR to generate vectors expressing the E2–p7–NS2 genes of HCV
1431F+KpnI |
5'-CGGGGTACCATGGTGGGGAACTGGGCGAAGGTCCTGG-3' |
3419Rter+EcoRI |
5'-CCGGAATTCTTACTACAGCAACCTCCACCCCTTGGAGACCAT-3' |
1900F |
5'-ACAGGTCGGGCGCGCCTACCTACAGCTGGG-3' |
2579R+15 |
5'-CACCTCCGTGTCCAGCGCCTCCGCTTGGGATATGAGTAAC-3' |
2769F+15 |
5'-TCCCAAGCGGAGGCGCTGGACACGGAGGTGGCCGCGTCGT-3' |
2989R |
5'-GTCGGGTGTACTACACACATGAGTAAGATG-3' |
2624R+15 |
5'-GAGCAGGAGGAGAGGCCCGGCCAGGGATGCTGCATTGAG-3' |
2724F+15 |
5'-GCATCCCTGGCCGGGCCTCTCCTCCTGCTCCTGCTGGCGTTG-3' |
2693R(M) |
5'-GGGCACCCAGCTACCTATCAGATACCACGCAAAGCA-3' |
2667F(M) |
5'-TGGTATCTGATAGGTAGCTGGGTGCCCGGAGCGGTC-3' |
E2ter+15 |
5'-GGAATTAATTCGCTACGCCTCCGCTTGGGATATGAG-3' |
terIRES+15 |
5'-CAAGCGGAGGCGTAGCGAATTAATTCCGGTTATTTT-3' |
IRES-R |
5'-TGGCCATGGTATTATCATCGTGTT-3' |
Sec-F |
5'-AATGGGAGTTTGTTTTGGCAC-3' |
Sec-R+15 |
5'-GAGGTTCTCCAAAGCGTCACCAGTGGAACCTGGAAC-3' |
p7NS2+15 |
5'-GGTTCCACTGGTGACGCTTTGGAGAACCTCGTAATA-3' |
p7-R+IRES |
5'-ACCGGAATTAATTCGCTATGCGTATGCCCGCTGAGGCAA-3' |
NS2-F+sig |
5'-GGTTCCACTGGTGACCTGGACACGGAGGTGGCCGCG-3' |
IRES-F+p7 |
5'-CAGCGGGCATACGCATAGCGAATTAATTCCGGTTATTTT-3' |
sig-R+NS2 |
5'-CACCTCCGTGTCCAGGTCACCAGTGGAACCTGGAAC-3' |
E2R-J6p7 |
5'-CAGCTTCTCTAGTGCCGCCTCCGCTTGGGATATGAG-3' |
NS2F-J6p7 |
5'-CAACAGGCTTATGCTCTGGACACGGAGGTGGCCGCG-3' |
J6p7-F |
5'-TCCCAAGCGGAGGCGGCACTAGAGAAGCTGGTCATC-3' |
J6p7-R |
5'-CACCTCCGTGTCCAGAGCATAAGCCTGTTGGGGCAA-3' |
N-tail-R |
5'-ATTGCAGCTAGCTGCGGATGCTGCATTGAGTATTAC-3' |
C-tail-F |
5'-CTCCTAGCATTGCCACAGCGGGCATACGCACTGGAC-3' |
N-tail-F |
5'-CTCAATGCAGCATCCGCAGCTAGCTGCAATGGCTTC-3' |
C-tail-R |
5'-TGCGTATGCCCGCTGTGGCAATGCTAGGAGCAGTAG-3' |
cyto-R |
5'-TCCGGGCACCCACCTACCCTTCAGGTACCAAGCAGCCACGAAAAA-3' |
cyto-F |
5'-CTGAAGGGTAGGTGGGTGCCCGGAGCTACCTATTCCCTCACTGGC-3' |
TMD-1R |
5'-CGCAGCGTGCAAGATGACCAGCTTCTCTAGTGCCGCCTCCGCTTGGGATATGAG-3' |
TMD-2F |
5'-CTAGAGAAGCTGGTCATCTTGCACGCTGCGAGCCTGGCCGGGACGCACGGTCTT-3' |
TMD-2R |
5'-TAAGGGGACTACCCGACCCTTGATATACCACGCAAAGCAGAAGAA-3' |
TMD-3F |
5'-ATCAAGGGTCGGGTAGTCCCCTTAGCGGTCTACGCCCTCTACGGG-3' |
TMD-3R |
5'-CACCTCCGTGTCCAGAGCATAAGCCTGTTGAGGCAACGCCAGCAGGAGCAG-3' |