Activation of the Flt3 signal transduction cascade rescues and enhances type I interferon-producing and dendritic cell development -- Data Supplement - JEM--Onai et al. -- The Journal of Experimental Medicine

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Figure S1. Diagrams of pMY-IRES-GFP and pMY-huFlt3-IRES-GFP bicistronic retroviral expression vector constructs and retroviral transduction efficacy. (A) Diagrams of pMY-IRES-GFP and pMY-huFlt3-IRES-GFP bicistronic retroviral expression vector constructs. LTRs, long terminal repeats; PCMV, PCC-4, cell-passaged myeloproliferative sarcoma virus; MPSV, myeloproliferative sarcoma virus; Ψ, packaging signal. (B) Histograms show typical GFP expression in Flt3^- and Flt3⁺ progenitor cells transduced with either pMYs-IRES-GFP or pMYs-huFlt3-IRES-GFP viruses for 18 h. Percentages of GFP⁺ cells are indicated.