[View Larger Version of this Image]

Figure S4. Analysis of FSAP mRNA by RT-PCR in mouse vascular cells and tissue. RNA was isolated from cells or from tissue using the RNeasy kit (Qiagen). After quantification of RNA, equal amounts were used for the reverse transcription reaction. The source sequence for the primer design was NM_146101 and the forward primer was in exon 9 and the reverse primer in exon 11 so that genomic DNA would not be amplified. Mouse β-actin was used as a positive control. Liver RNA was used as a positive control, and water was used as a negative control.