Cell populations in bone marrow

Number of cells (×10⁶)

WT

puma^-/-

bim^-/-

bim^-/-/puma^-/-

bcl-2 tg

P-values

Total bome marrow
 cellularity, two femora

 34.85
(± 2.99)

 29.18
(± 3.66)

 33.18
(± 2.48)

  28.44
  (± 4.63)

 50.83
(± 9.09)

bcl-2 tg vs other genotypes
 P ≤ 0.0486

B220⁺CD43⁺sIgM^-
 pro–B cells

 1.53
(± 0.14)

 1.36
(± 0.21)

 2.97
(± 0.15)

  2.68
  (± 0.54)

 5.76
(± 0.97)

WT vs bim^-/- and DKO
 P ≤ 0.0219
bcl-2 tg vs bim^-/- and DKO
 P ≤ 0.0176

B220⁺CD43^-sIgM^-
 pre–B cells

 4.42
(± 0.68)

 3.12
(± 0.47)

 3.66
(± 0.58)

  2.52
  (± 0.40)

 4.23
(± 0.97)

WT vs DKO
 P = 0.0819

B220⁺sIgM⁺ B cells

 4.02
(± 0.48)

 3.07
(± 0.31)

 6.32
(± 0.47)

  5.49
  (± 1.14)

 13.89
(± 4.49)

WT vs bim^-/-
 P = 0.0029
bcl-2 tg vs bim^-/-
 P = 0.031

sIgM^lowsIgD^high B cells

 1.18
(± 0.40)

 1.62
(± 0.36)

 2.83
(± 0.34)

  1.95
  (± 0.41)

 7.22
(± 3.06)

WT vs bim^-/- and bcl-2 tg
 P ≤ 0.021

CD4⁺ T cells

 0.54
(± 0.13)

 0.60
(± 0.08)

 1.23
(± 0.16)

  2.26
  (± 0.22)

 5.03
(± 1.35)

WT vs bim^-/-
 P = 0.0087
bim^-/- vs DKO
 P = 0.005
DKO vs bcl-2 tg
 P = 0.0897

CD8⁺ T cells

 0.52
(± 0.13)

 0.42
(± 0.06)

 1.26
(± 0.15)

  1.90
  (± 0.61)

 1.45
(± 0.38)

WT vs bim^-/-, DKO, and bcl-2 tg
 P ≤ 0.0172

Nucleated Ter119⁺
 erythroid cells

 9.89
(± 1.37)

 8.67
(± 2.53)

 6.34
(± 0.57)

  4.60
  (± 0.43)

 6.92
(± 1.44)

WT vs bim^-/- and DKO
 P ≤ 0.0201

Gr-1⁺ granulocytes

 8.83
(± 0.70)

 8.06
(± 0.68)

 5.72
(± 0.78)

  3.73
  (± 0.77)

 5.72
(± 1.56)

WT vs other genotypes
 P ≥ 0.258

Mac-1⁺Gr-1^- monocytes

 3.90
(± 1.09)

 2.12
(± 0.39)

 3.13
(± 0.48)

  2.36
  (± 0.67)

 5.97
(± 1.52)

WT vs bim^-/- and DKO
 P ≤ 0.0078

Bone marrow samples from animals of the indicated genotypes were obtained from both femora. Aliquots of single-cell suspensions were counted in a hemocytometer using trypan blue to determine the overall cellularity. Total numbers of the different hemopoietic subpopulations were determined by staining aliquots with fluorescence-conjugated antibodies specific for the myeloid markers Mac-1 and Gr-1, the erythroid cell marker Ter-119, the T cell markers CD4 and CD8, or the B cell markers B220, sIgM, and CD43 followed by flow cytometric analysis. Means ± SEM of 5–10 animals per genotype are given. DKO, double knockout.