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Figure S1. YF-17D induces IL-12p40 and IL-12p70 in both human mDCs (Fig. 1, A and B) and murine splenic CD11c⁺ DCs (Fig. 1 C). Induction of IL-12p70 was only observed when an additional CD40-L signal was provided, in the form of a fibroblast cell line expressing CD40-L (Triantafilou, K., G. Orthopoulos, E. Vakakis, M.A. Ahmed, D.T. Golenbock, P.M. Lepper, and M. Triantafilou. 2005. Cell. Microbiol. 7:1117-1126), consistent with numerous previous studies (Dillon, S., A. Agrawal, T. Van Dyke, G. Landreth, L. McCauley, A. Koh, C. Maliszewski, S. Akira, and B. Pulendran. 2004. J. Immunol. 172:4733-4743). Note that induction of IL-12p70 by CD40-L stimulation alone resulted in little or no IL-12p70.